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Indeed, cell-mediated immunity requires that protein antigens be first degraded in the endocytic vesicles of APCs (eg, macrophages, dendritic cells), so that the smaller peptides can be presented on the cell surface by the major histocompatibility complex to activate proliferation of antigen-specific T cells

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The absorbance was measured at 450 nm using a Varioskan LUX microplate reader (Thermo Fisher Scientific, MA, USA)